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Registry of Toxic Effects of Chemical Substances (RTECS)

1,3-Butadiene, hexachloro-

RTECS #

EJ0700000

CAS #

87-68-3

Updated

September 201

Molecular Weight

260.74

Molecular Formula

C4Cl6

Synonyms

1,1,2,3,4,4-Hexachloro-1,3-butadiene
1,3-Hexachlorobutadiene
Dolen-pur
GP-40-66:120
HCBD
Hexachlor-1,3-butadien (Czech)
Hexachlorbutadiene
Hexachloro-1,3-butadiene
Hexachlorobutadiene
Hexachlorobutadiene (ACGIH)
Perchlorobutadiene
RCRA waste number U128

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 162 mg mildEJTXAZ 9,171,1976
eye /rabbit 500 mg/24H mild85JCAE -,116,1986
skin /rabbit 810 mg/24H moderateEJTXAZ 9,171,1976
skin /rabbit 500 mg/24H mild85JCAE -,116,1986

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysislymphocyte/human1 mg/LCYGEDX 22(4),46,1988
Cytogenetic Analysisoral/mouse2 mg/kgCYGEDX 22(4),46,1988
Cytogenetic Analysisinhalation/mouse10 mg/m3CYGEDX 22(4),46,1988
Cytogenetic Analysislung/hamster6.3 mg/L/4H (+/-enzymatic activation step)MUREAV 699,47,2010
DNA Damageliver/human10 µmol/l/48HMUREAV 696,21,2010
DNA Damageliver/human1 mmol/l/48HMUREAV 696,21,2010
DNA Damageliver/human1 µmol/l/48HMUREAV 696,21,2010
morphological transformembryo/hamster10 mg/LCALEDQ 23,297,1984
mutation in microorganisms/Salmonella typhimurium1 mg/plate (+enzymatic activation step)DHEFDK FDA-78-1046,1978
mutation in microorganisms/Salmonella typhimurium320 µg/plate (-enzymatic activation step)CRNGDP 7,431,1986
sister chromatid exchangeovary/hamster1400 µg/LEMMUEG 10(Suppl 10),1,1987
Unscheduled DNA Synthesisembryo/hamster2 mg/LCALEDQ 23,297,1984
Unscheduled DNA Synthesisoral/rat77 gm/kg/11WTXAPA9 60,287,1981

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
inhalation/rat 15 ppm/6H (6-20D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)TOLED5 47,235,1989
intraperitoneal/rat 150 mg/kg (1-15D pregnant)Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)

Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)		SWEHDO 7(Suppl 4),66,1981
oral/rat 45 mg/kg (13W male/13W pre-3W after birth)Reproductive: Effects on newborn: Biochemical and metabolicTXAPA9 42,387,1977
oral/rat 4 gm/kg (13W male/13W pre-3W after birth)Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)TXAPA9 42,387,1977
oral/rat 178 mg/kg (1-22D pregnant)Reproductive: Specific developmental abnormalities: Central nervous system

Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)		AAGEAA 89(8),44,1985
oral/mouse 10920 µg/kg (13W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)NTIS** PB91-185884
oral/rat 20 mg/kg (multigenerations)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)NTIS** #PB93-123867
oral/rat 75 mg/kg (multigenerations)Reproductive: Specific developmental abnormalities: Urogenital system

Reproductive: Effects on newborn: Biochemical and metabolic		NTIS** #PB93-123867
oral/rat 225 mg/kg (17-22D pregnant)Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth)NTIS** #PB93-123867
oral/rat 37.5 mg/kg (multigenerations)Reproductive: Effects on newborn: Viability index (e.g., # alive at day 4 per # born alive)

Reproductive: Effects on newborn: Biochemical and metabolic		NTIS** #PB93-123867
oral/rat 5 mg/kg (multigenerations)Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)NTIS** #PB93-123867
subcutaneous/rat 20 mg/kg (1D prior to copulation)Reproductive: Effects on newborn: Weaning or lactation index (e.g., # alive at weaning per # alive at day 4)GISAAA 31,33,1966

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/rat lowest published toxic dose: 15 gm/kg/2Y- continuousTumorigenic: Carcinogenic by RTECS criteria

Kidney, Ureter, and Bladder: Kidney tumors		AIHAAP 38,589,1977

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
inhalation/mammal (species unspecified) lethal concentration (50 percent kill): 370 mg/m3GISAAA 55(5),72,1990
inhalation/mouse lethal concentration (50 percent kill): 370 mg/m3GISAAA 37(2),32,1972
inhalation/mouse lethal concentration (50 percent kill): 370 mg/m3/2HVCVGH* -,518,1990
inhalation/rat lethal concentration (50 percent kill): 630 mg/m3/4HVCVGH* -,518,1990
intraperitoneal/mouse lethal dose (50 percent kill): 76 mg/kgEJTXAZ 7,247,1974
intraperitoneal/mouse lowest published toxic dose: 25 mg/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)VCVGH* -,519,1990
intraperitoneal/rat lowest published toxic dose: 300 mg/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Other changes in urine composition		VCVGH* -,519,1990
intraperitoneal/rat lethal dose (50 percent kill): 175 mg/kgBehavioral: Somnolence (general depressed activity)

Behavioral: Irritability		EJTXAZ 8,180,1975
intraperitoneal/rat lowest published toxic dose: 100 mg/kgKidney, Ureter, and Bladder: Other changesTOLED5 172S,S1,2007
intraperitoneal/rat lowest published toxic dose: 100 mg/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Renal function tests depressed		TOLED5 172S,S1,2007
intraperitoneal/rat lowest published toxic dose: 50 mg/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)TOLED5 182,84,2008
intraperitoneal/rat lowest published toxic dose: 100 mg/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Metabolism (intermediary): Other proteins		TOLED5 182,84,2008
oral/cat lethal dose (50 percent kill): 187 mg/kgVCVGH* -,519,1990
oral/guinea pig lowest published toxic dose: 10 mg/kgNutritional and Gross Metabolic: Conditioned vitamin deficiencyVCVGH* -,519,1990
oral/guinea pig lethal dose (50 percent kill): 90 mg/kgENTOX* -,513,2005
oral/hamster lethal dose (50 percent kill): 960 mg/kgTXAPA9 48,A192,1979
oral/mouse lethal dose (50 percent kill): 51 mg/kgVCVGH* -,518,1990
oral/rat lethal dose (50 percent kill): 82 mg/kgGISAAA 37(2),32,1972
oral/rat lowest published toxic dose: 8.1 mg/kgSpinal Cord: Other degenerative changesVCVGH* -,520,1990
oral/rat lowest published toxic dose: 10 mg/kgNutritional and Gross Metabolic: Conditioned vitamin deficiencyVCVGH* -,519,1990
oral/rat lowest published toxic dose: 200 mg/kgKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Other changes in urine composition

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Peptidases		TXAPA9 132,203,1995
skin/mouse lethal dose (50 percent kill): 150 mg/kgVCVGH* -,518,1990
skin/rabbit lethal dose (50 percent kill): 100 mg/kgGISAAA 37(2),32,1972
skin/rabbit lethal dose (50 percent kill): 126 mg/kg/7HENTOX* -,513,2005
skin/rat lethal dose (50 percent kill): 4500 mg/kgVCVGH* -,519,1990
unreported route/mammal (species unspecified) lethal dose (50 percent kill): 200 mg/kg30ZDA9 -,40,1971
unreported route/rat lowest published toxic dose: 200 mg/kgKidney, Ureter, and Bladder: Other changes in urine compositionTOXID9 44,104,2005

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/man lowest published toxic concentration: 0.02 ppm/9Y- intermittentLiver: Change in gall bladder structure or function

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)		BJIMAG 49,700,1992
inhalation/rat lowest published toxic concentration: 26 mg/m3/2H/30D- intermittentBlood: Other changes

Blood: Changes in leukocyte (WBC) count

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		GISAAA 37(2),32,1972
inhalation/rat lowest published toxic concentration: 100 ppm/6H/2W- intermittentKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field		BJIMAG 27,1,1970
intraperitoneal/rat lowest published toxic dose: 50 mg/kg/2D- intermittentKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Renal function tests depressed

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases		CBCPAI 136,367,2003
intraperitoneal/rat lowest published toxic dose: 75 mg/kg/3D- intermittentBehavioral: Tolerance

Kidney, Ureter, and Bladder: Other changes

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field		CBCPAI 136,367,2003
intraperitoneal/rat lowest published toxic dose: 100 mg/kg/4D- intermittentBehavioral: Ataxia

Behavioral: Tolerance

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field		CBCPAI 136,367,2003
intraperitoneal/rat lowest published toxic dose: 313 mg/kg/3D- intermittentLiver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Phosphatases		TIHEEC 8,191,1992
oral/guinea pig lowest published toxic dose: 420 mg/kg/30W- intermittentLiver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Immunological Including Allergic: Increase in cellular immune response		GTPZAB 9(11),50,1965
oral/mouse lowest published toxic dose: 540 mg/kg/15D- continuousCardiac: Changes in heart weight

Endocrine: Changes in spleen weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		JEPOEC 9,323,1989
oral/mouse lowest published toxic dose: 1092 mg/kg/13W- continuousCardiac: Changes in heart weight

Kidney, Ureter, and Bladder: Changes in bladder weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		NTIS** PB91-185884
oral/rat lowest published toxic dose: 1274 mg/kg/26W- intermittentBrain and Coverings: Other degenerative changes

Behavioral: Alteration of operant conditioning

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)		GTPZAB 11(3),23,1967
oral/rat lowest published toxic dose: 14600 mg/kg/2Y- continuousKidney, Ureter, and Bladder: Other changes in urine composition

Kidney, Ureter, and Bladder: Other changes

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		EVHPAZ 21,49,1977
oral/rat lowest published toxic dose: 105 mg/kg/14D- continuousKidney, Ureter, and Bladder: Changes in bladder weight

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		TXAPA9 47,1,1979
oral/rat lowest published toxic dose: 794 mg/kg/18W- intermittentKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Changes in bladder weight

Liver: Changes in liver weight		TXAPA9 47,1,1979
oral/rat lowest published toxic dose: 80 mg/kg/16D- continuousBehavioral: Food intake (animal)

Kidney, Ureter, and Bladder: Other changes

Nutritional and Gross Metabolic: Weight loss or decreased weight gain		NTIS** #PB93-123867
oral/rat lowest published toxic dose: 160 mg/kg/16D- continuousBehavioral: Tremor

Behavioral: Muscle weakness		NTIS** #PB93-123867
oral/rat lowest published toxic dose: 160 mg/kg/16D- continuousBehavioral: SleepNTIS** #PB93-123867
oral/rat lowest published toxic dose: 160 mg/kg/16D- continuousKidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)

Kidney, Ureter, and Bladder: Changes in kidney weight		NTIS** #PB93-123867
oral/rat lowest published toxic dose: 1040 mg/kg/16D- continuousKidney, Ureter, and Bladder: Other changes in urine compositionNTIS** #PB93-123867
oral/rat lowest published toxic dose: 320 mg/kg/16D- continuousBehavioral: Fluid intakeNTIS** #PB93-123867
oral/rat lowest published toxic dose: 600 mg/kg/16D- continuousKidney, Ureter, and Bladder: Urine volume increasedNTIS** #PB93-123867
oral/rat lowest published toxic dose: 385 mg/kg/7D- continuousRelated to Chronic Data: Death in the "MULTIPLE DOSE" data type fieldNTIS** #PB93-123867
oral/rat lowest published toxic dose: 560 mg/kg/4W- continuousKidney, Ureter, and Bladder: Other changes in urine composition

Endocrine: Changes in adrenal weight

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Transaminases		FCTOD7 31,125,1993

Reviews

OrganizationStandardReference
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-time-weighted average 0.02 ppm (skin)DTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-Confirmed animal carcinogenDTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC)Cancer Review:Animal Limited EvidenceIMEMDT 20,179,1979
International Agency for Research on Cancer (IARC)Cancer Review:Animal Limited EvidenceIMEMDT 73,277,1999
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 20,179,1979
International Agency for Research on Cancer (IARC)Cancer Review:Human Inadequate EvidenceIMEMDT 73,277,1999
International Agency for Research on Cancer (IARC)Cancer Review:Group 3IMEMDT 73,277,1999
TOXICOLOGY REVIEWENTOX* -,513,2005
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWBCLPT* 96,131,2005
TOXICOLOGY REVIEWMUREAV 590,1,2005

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIAtime-weighted average 0.02 ppm (0.21 mg/m3), Carcinogen, JUL2008
Occupational Exposure Limit-BELGIUMtime-weighted average 0.02 ppm (0.21 mg/m3), Skin, MAR2002
Occupational Exposure Limit-DENMARKtime-weighted average 0.02 ppm (0.24 mg/m3), skin, MAY2011
Occupational Exposure Limit-ICELANDtime-weighted average 0.02 ppm (0.24 mg/m3), skin, NOV2011
Occupational Exposure Limit-KOREAtime-weighted average 0.02 ppm (0.24 mg/m3), skin, 2006
Occupational Exposure Limit-NEW ZEALANDtime-weighted average 0.02 ppm (0.21 mg/m3), skin, JAN2002
Occupational Exposure Limit-NORWAYtime-weighted average 0.02 ppm (0.24 mg/m3), JAN1999
Occupational Exposure Limit-PERUtime-weighted average 0,02 ppm (0,21 mg/m3), JUL2005
Occupational Exposure Limit-RUSSIAshort term exposure limit 0.005 mg/m3, Skin, JUN2003
Occupational Exposure Limit-SWITZERLANDMAK-week 0.02 ppm (0.24 mg/m3), carc 3, skin, JAN2011
Occupational Exposure Limit-THE NETHERLANDSMAC-TGG 0.24 mg/m3, 2003

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO HEXACHLOROBUTADIENE-air10H Carcinogen time-weighted average 0.02 ppm (Sk)NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code X6278; Number of Industries 1; Total Number of Facilities 3; Number of Occupations 11; Total Number of Employees Exposed 1010; Total Number of Female Employees Exposed 26

Status in Federal Agencies

OrganizationReference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/95/100160/AS)
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULEFEREAC 47,26992,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Hexachlorobutadiene, 2543
NTP Toxicity studies, RPT# TOX-01, October 2000
On EPA IRIS database

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