The PFGE Process

The Pulsed-field Gel Electrophoresis Process: (1)The scientist takes bacterial cells from an agar plate; (2)the scientist mixes bacterial cells with melted agarose before DNA is extracted from them; (3)the scientist loads the DNA gelatin plug into a gel, and (4)the gel is stained so that DNA can be seen under UV light.

The MLVA Process

MLVA Work Flow: Specimen, Culture growth, DNA Template, PCR set up and amplification, Fragment analysis set, Capillary electrophoresis (CEQ and ABI), Peak file, and Allele assignment.

The Whole Genome Sequencing (WGS) Process

Whole Genome Sequencing Process - WGS is a laboratory procedure that determines the order of bases in the genome of an organism in one process. WGS provides a very precise DNS fingerprint that can help link cases to one another allowing an outbreak to be detected and solved sooner.

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Page last reviewed: May 29, 2013
Page last updated: February 14, 2014
Content source:
- National Center for Emerging and Zoonotic Infectious Diseases (NCEZID)
  , Division of Foodborne, Waterborne, and Environmental Diseases (DFWED)

Related Links

The PFGE Process

The MLVA Process

The Whole Genome Sequencing (WGS) Process

Related Links

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