Identification of other Streptococcus Species: Streptococcus General Methods
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The reference used for compiling the methods in Section I is:
Murray, P.R., Baron, E. J., Jorgensen, J.J., Pfaller, M.A., and Yolken, R.H. Manual of Clinical Microbiology, 8th ed. ASM Press: Washington, DC, 2003.
The Streptococcus species identification methods in Section II were compiled by several individuals, including Lynn Shewmaker, Richard Facklam, and Lucia Teixeira.
Section I.
- Accuprobe-Enterococcus Test
- Accuprobe-Pneumococcus Test
- Acid formation in carbohydrate broth
- Arginine Hydrolysis
- Bacitracin Test
- Bile-esculin Test
- Bile solubility Test
- CAMP Test
- Catalase Test
- Clindamycin test
- Esculin hydrolysis
- Gas from MRS broth
- Gram Stain
- Growth at 10 & 45C
- Hemolysis
- Hippurate hydrolysis
- Lancefield Group Antigen
- Leucine amino peptidase (LAP)
- Litmus Milk Test
- Motility
- 6.5% NaCl Tolerance Test
- Optochin
- Pigmentation
- Pyridoxal Requirement Test (Vitamin B6)
- Pyrrolidonlarylamindase (PYR)
- Pyruvate Utilization Test
- Satellite Test
- Starch hydrolysis
- Sucrose agar (extracellular polysaccharides)
- Sucrose broth (5%)
- Tellurite tolerance
- Urea hydrolysis
- Vancomycin test
- Voges-Proskauer
Section II.
Identification Procedures for Catalase-Negative Gram-positive Cocci
A. General Description of the general identified in this lab
- Table 1.
- Identification of gram-positive general Beta-hemolytic streptococci
- Table 2.
- Identification and reporting of β-hemolytic streptococci
- Table 3.
- Identification of atypical group A strep
- Table 4.
- Identification of the S. anginous group
Non-beta-hemolytic streptococci
Streptococcus pneumoniae - Table 5.
- Identification non-β-hemolytic strep
- Table 6.
- Characteristics of viridans streptococci
- Table 7.
- Identification of nutritionally variant strep
B. Identification of Enterococcus and Vagococcus
- Table 8.
- Identification of Enterococcus and Vagococcus species
C. Identification of Lactococcus species
- Table 9.
- Identification of Lactococcus species
D. Identification of Gemella species
- Table 10.
- Identification of Gemella species
E. Identification of Pediococcus and Tetragenococcus species
- Table 11.
- Identification of Pediococcus and Tetragenococcus
F. Identification of Leuconostoc species
- Table 12.
- Identification of Leuconostoc species
G. Identification of Aerococcus and Helococcus
- Table 13.
- Identification of Aerococcus and Helococcus
H. Identification of Globicatella
- Table 14.
- Identification of Globicatella
I. Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus
- Table 15.
- Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus
- Table 16.
- Rapid ID-32 for unusual gram positive bacteria
- Page last reviewed: March 26, 2014
- Page last updated: March 26, 2014
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